生活污水高效培养小球藻的环境优化

兰州理工大学学报 ›› 2020, Vol. 46 ›› Issue (4): 73-78.

生活污水高效培养小球藻的环境优化

王昱^1,2, 时文强^1,2, 郭亚敏^1,2, 卢世国^1,2, 刘娟娟^1,2

1.兰州理工大学能源与动力工程学院, 甘肃兰州 730050;
2.兰州理工大学甘肃省生物质能与太阳能互补供能系统重点实验室, 甘肃兰州 730050

收稿日期:2018-09-21 出版日期:2020-08-28 发布日期:2020-11-10
作者简介:王昱(1979-),男,甘肃永昌人,博士,副教授.
基金资助:
国家自然科学基金(51669011),甘肃省重大研发计划(18JR4RA002)

Environmental optimization of high-efficiency cultivation of Chlorella in domestic sewage

WANG Yu^1,2, SHI Wen-qiang^1,2, GUO Ya-min^1,2, LU Shi-guo^1,2, LIU Juan-juan^1,2

1. College of Energy and Power Engineering, Lanzhou Univ. of Tech., Lanzhou 730050, China;
2. Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou Univ. of Tech., Lanzhou 730050, China

Received:2018-09-21 Online:2020-08-28 Published:2020-11-10

摘要/Abstract

摘要： 为了在我国北方地区村镇用生活污水高效培养小球藻,对小球藻的生长环境(初始污水浓度、环境温度、光照强度和光照时间)进行了单因素和正交实验研究.结果表明:在单因素环境条件下,污水的初始污水浓度、环境温度、光照强度和光照时间对小球藻生长具有显著影响.最佳的初始污水浓度是将原生活污水稀释2倍,环境温度达到30 ℃,光照强度为12 000 Lux,光照时间为20 h.正交实验研究表明,生活污水培养小球藻的最优培养条件并非各环境因子最佳水平的简单叠加,而是受环境因子间耦合作用的控制,主导性环境因子是初始污水浓度和光照时间.将原生活污水稀释2倍、环境温度为30 ℃、光照强度为10 000 Lux、光照时间达16 h,此时的生活污水培养小球藻的效率最高.

关键词: 生活污水, 小球藻, 环境因子

Abstract: In order to cultivate Chlorella effectively with domestic sewage in villages and towns in northern China, the growth environment (initial sewage concentration, ambient temperature, illumination intensity and illumination time) for Chlorella was studied by single factor and orthogonal experiments in this study. The results showed that the initial sewage concentration, ambient temperature, illumination intensity and illumination time of sewage have respectively a significant effect on the growth of Chlorella under single-factor environmental conditions. The optimum initial sewage concentration is two times dilution of raw sewage, an ambient temperature of 30 ℃, an illumination intensity of 12 000 Lux, and a illumination time of 20 h. The results of orthogonal experiments showed that the optimal culture conditions of Chlorella are not a simple overlay of environmental factors, but controlled by the coupling of environmental factors. The dominant environmental factors are the initial sewage concentration and illumination time. Two times dilution of the raw sewage, the ambient temperature of 30 ℃, the illumination intensity of10 000 Lux, the illumination time of 16 h, the sewage culture Chlorella reaches the highest efficiency at this time.

Key words: domestic sewage, Chlorella, environmental factors

中图分类号:

王昱, 时文强, 郭亚敏, 卢世国, 刘娟娟. 生活污水高效培养小球藻的环境优化[J]. 兰州理工大学学报, 2020, 46(4): 73-78.

WANG Yu, SHI Wen-qiang, GUO Ya-min, LU Shi-guo, LIU Juan-juan. Environmental optimization of high-efficiency cultivation of Chlorella in domestic sewage[J]. Journal of Lanzhou University of Technology, 2020, 46(4): 73-78.

参考文献

[1] CHI Z Y,ZHENG Y B,JIANG A P,et al.Lipid production by culturing oleaginous yeast and algae with food waste and municipal wastewater in an integrated process [J].Appl Biochem Biotechnol,2011,165(2):442-453.
[2] DEMIRBAS M F.Biofuels from algae for sustainable development [J].Appl Energy,2011,88(10):3473-3480.
[3] 胡洪营,李鑫,杨佳.基于微藻细胞培养的水质深度净化与高价值生物质生产耦合技术 [J].生态环境学报,2009,18(3):1122-1127.
[4] 钟成华,周晓琴,苏翔,等.利用市政污水培养产油微藻的研究 [J].安全与环境学报,2014,14(6):157-160.
[5] SHAHAT A A,COS P,DE BRUYNE T,et al.Antiviral and antioxidant activity of flavonoids and proanthocyanidins from Crataegus sinaica [J].Planta Med,2002,68(6):539-541.
[6] GARCIA J,GREEN B F,LUNDQUIST T,et al.Long term diurnal variations in contaminant removal in high rate ponds treating urban wastewater [J].Bioresource Technology,2006,97(14):1709-1715.
[7] CRAGGS R J,DAVIES-COLLEY R J,TANNER C C,et al.Advanced pond system:performance with high rate ponds of different depths and areas [J].Water Science and Technology,2003,48(2):259-267.
[8] El H F,El H B.Anaerobic reactor/high rate pond combined technology for sewage treatment in the mediterranean area [J].Water Science and Technology,2005,51(12):125-132.
[9] HU B,ZHOU W G,MIN M,et al.Development of an effective acidogenically digested swine manure-based algal system for improved wastewater treatment and biofuel and feed production [J].Applied Energy,2013,107(3):255-263.
[10] 张桂艳,温小斌,梁芳,等.重要理化因子对小球藻生长和油脂产量的影响 [J].生态学报,2011,31(8):2076-2085.
[11] 卢碧林,祁亮,李明习.光温培养条件对小球藻Chlorella sp.生长及产物的影响 [J].可再生能源,2014,32(10):1527-1533.
[12] JOHN B.The cyanobacteria isolation purification and identification [J].Prokaryotes,2006(4):1053-1073.
[13] 韩松芳,金文标,涂仁杰,等.基于城市污水资源化的微藻筛选与污水预处理 [J].环境科学,2017,38(8):3347-3353.
[14] CAI T,GE X,PARK S Y,et al.Comparison of Synechocystis sp.PCC6803 and Nannochloropsis salina for lipid production using artificial seawater and nutrients from anaerobic digestion effluent [J].Bioresource Technology,2013,144(5):255-260.
[15] PARK J,JIN H F,LIM B R,et al.Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp.[J].Bioresoure Technology,2010,101(22):8649-8657.
[16] 邓光,李夜光,胡鸿钧,等.温度、光照和pH值对锥状斯氏藻和塔玛亚力山大藻光合作用的影响及光暗周期对其生长速率和生物量的影响 [J].武汉植物学研究,2004,22(2):129-135.
[17] 欧阳峥嵘,温小斌,耿亚红,等.光照强度、温度、pH、盐度对小球藻(Chlorella)光合作用的影响 [J].武汉植物学研究,2010,28(1):49-55.
[18] GU B H,ALEXANDER V.Seasonal variations in dissolved inorganic nitrogen utilization in a subarctic Alaskan lake [J].Arch Hydrobiol,1993,126(3):273-288.
[19] 王志红,崔福义,安全.水温与营养值对水库藻华态势的影响 [J].生态环境,2005(1):10-15.
[20] UGWU C U,AOYAG H,UCHIYAMA H.Influence of irradiance,dissolved oxygen concentration and temperature on the growth of Chlorella sorokiniana [J].Photosynthetica,2007,45(2):309-311.
[21] 杨桂娟,栾忠奇,周笑辉.温度对小球藻生长量和溶氧量影响研究 [J].农机化研究,2009,31(9):157-158.
[22] 吕福荣,杨海波,李英敏,等.自养条件下小球藻净化氮磷能力的研究 [J].生物技术,2003(6):46-47.
[23] 杨佩昀,徐婷婷,高伟.温度、光照和磷质量浓度对小环藻、大型溞和金鱼藻共培养的影响 [J].河南师范大学学报(自然科学版),2016,44(1):110-116.